buttery-eel
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Psy-Fer
The buttery eel - a slow5 guppy/dorado basecaller wrapper
I could successfully setup buttery-eel on my MacBook Air, which has the Apple M2 chip and is running macOS Sonoma version 14.1.1. Below are the commands I used for the...
sometimes a run will crash or hit a wall limit due to hardware of system limits. It would be helpful to easily resume the sequencing run. I think a quick...
Hello, I'm trying to basecall some reads I generated with squigulator. `buttery-eel -i results/zymo-human/passed-1K.blow5 -o results/zymo-human/passed-1K-squigulator.fastq -x cuda:all -g resources/ont-guppy-6_5_7/bin/ --config dna_r10.4.1_e8.2_400bps_sup.cfg --port 8020 --slow5_threads 128` When trying to basecall...
Hi, I encountered an issue when using buttery-eel to baseclling with dorado-server.  I want to use Dorado to basecall the RNA methylation model. Here is my code: ` Slow5Dir="/public2/hjliang/ONT_data/slow5"...
Thank you for the excellent program. I am currently testing the latest version with several parameters for ONT ultra-long read datasets. However, I noticed a potential omission in usage options...
Progress shows a snapshot of chunked basecalled reads It would be good I it showed the full total at the ned as well 
Here are the versions I used ``` dorado_basecall_server --version Dorado Basecall Service Software, (C)Oxford Nanopore Technologies plc. Version 7.1.4+d7df870c0, client-server API version 15.0.0 buttery-eel --version buttery-eel - wrapping guppy for...
The issue presented as Buttery-eel saying it is doing sup basecalling when it was supposed to be doing hac basecalling for me: ``` timamo@brenner-fpga:PSXPX230069:$ qstat -j 4204295 | grep -e...
Please add the following instructions to somewhere: For FASTQ output from buttery-eel, we call guppy_barcoder which comes with the ONT Guppy package for demuxing. It seems that guppy_barcoder does not...
Allow a user to provide a list of read-ids to basecall. This will then use random access get calls to pyslow5