buttery-eel
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Gets many reads skipped with dorado
Here are the versions I used
dorado_basecall_server --version
Dorado Basecall Service Software, (C)Oxford Nanopore Technologies plc. Version 7.1.4+d7df870c0, client-server API version 15.0.0
buttery-eel --version
buttery-eel - wrapping guppy for SLOW5 basecalling version: 0.3.3
When methylation calling using the following command,
buttery-eel -g ont-dorado-server/bin --config dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup_prom.cfg -i PGXX22394_reads_chr22.blow5 -o out.sam --call_mods --port 5557
I get the following message for around 50 different read IDs
Skipped a read: {read-id}
This did not happen with guppy+buttery-eel. I appreciate it if you could take a look. Thank you