Key error - 'mean_qscore'

[denisbeslic]

Hello,

I'm trying to basecall some reads I generated with squigulator.

buttery-eel -i results/zymo-human/passed-1K.blow5 -o results/zymo-human/passed-1K-squigulator.fastq -x cuda:all -g resources/ont-guppy-6_5_7/bin/ --config dna_r10.4.1_e8.2_400bps_sup.cfg --port 8020 --slow5_threads 128

When trying to basecall with buttery-eel I get the following error: An exception occurred: KeyError - 'mean_qscore' The fastq file is empty.

The header of the fastq for the input of squigulator looked like this: >81438ac8-1858-44d9-a8de-cec4e7e26adc_parent_read_id=81438ac8-1858-44d9-a8de-cec4e7e26adc_model_version_id=dna_r10.4.1_e8.2_400bps_sup-v4.1.0_mean_qscore=6 I removed the additional information such that the header looks like this: >81438ac8-1858-44d9-a8de-cec4e7e26adc_parent_read_id=81438ac8-1858-44d9-a8de-cec4e7e26adc However, I still get the same error.