LanSabb
LanSabb
Hi I am currently dealing with Pacbio Hifi metagenomes Do you think tha CoverM is suitable to compute the coverage for the long-read sequence? Thanks
Hi Genomad produces my test-run result I am likely to deem the contigs as viral sequences if virus_score > 0.95, however most of the results were fully lacked of viral...
Hi I installed the cblaster and attempted to build the local DB with the following code mamba create -y -n cblaster cblaster=1.3.20 ; conda activate cblaster (Input folder contains 14...
Hi I am attempting to run semibin2 with the following command : SemiBin2 single_easy_bin -t 80 -i Contigs.fa -b Contigs.bam -o Result -environment global However, the following error is repeated....
Hi. I am running semibin used as a module in the Pacbio HiFi-MAG-Pipeline (https://github.com/PacificBiosciences/pb-metagenomics-tools/blob/master/docs/Tutorial-HiFi-MAG-Pipeline.md) The snakemake pipeline automatically performs binning (FYI, the command looks like this) In="Read.incomplete_contigs.fasta" In2="Read.bam" Out="Semibin2" ;...
Hi I would like to ask how to interpret outputs I want to confirm whether my MAGs are complete or circular GCI seems to be an index to evaluate the...
Hi Thanks for nice tool I want to cluster the contigs assembled using short- and long-reads from multiple tools I attempted to assemble contigs using other tools (I will not...
Hi I am attempting to dereplicate the reconstructed MAGs at species level using following commands In="CheckM2 output" In2="Folder possessing MAGs"/* ; Out="Output folder" ; mkdir -p $Out ; dRep dereplicate...
Hi. I have an inquiry on result interpretation Image is example of binndash output Does Jacard index is proportion of values in column 5 (e.g. 0/2048 = 0. so 0...
Hi. Thanks for nice tool I would like to ask the vOTU clustering. I have large multi-contig viral sequences including both putative giant viruses and vOTUs not affiliated to putative...