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Hi, Thanks for replying me. Yes, I found that lowering the cutoff 2M doesn't introduce more duplicated sequence. So it's fine. But I recently realised that those places that can't...

Hi, I have the same issue with the file limits. We have > 2,700,000 reads in a PromthION run for one sample. In order to use the `tombo preprocess annotate_raw_with_fastq`...

Ok, thanks for replying me!

Thanks a lot for replying me so fast! I found some error in my completeness calculation. So the 70% completeness is due to the wrong file used for data base...

Thanks for the fast reply! I tried the line you suggested, but got the same error as before...