Liang Ou

Thank you for your support and look forward to the launch of the new version. Wish you safety and get through the hard time.

Thanks,the missing of `--recursive` is the reason of trouble.

Hi: I had a similar problem again. ``` self.handle = h5py.File(self.filename, self.mode) File "/home/anaconda3/lib/python3.7/site-packages/h5py/_hl/files.py", line 394, in __init__ swmr=swmr) File "/home/anaconda3/lib/python3.7/site-packages/h5py/_hl/files.py", line 170, in make_fid fid = h5f.open(name, flags, fapl=fapl)...

Thanks for your help. @myrtlecat I used `lsof `and `fuser` to make sure that the folder was not being occupied by another process. On the other hand,The` OSError` didn't appear...

supplementary question： Does the decline of the loss curve indicate that my training is effective and correct？

Hi: I found that there was a big difference between the original sequence(Generated during Gridion sequencing) and the sequence generated by the modified base model. here is an example. ```...

> loss in mapped squiggle for RNA reads is also compounded by polyA absent from guppy called fastq. So you have encountered a similar situation?My friend:) > I guess the...

Thanks a lot!!!I will try to alter the parameter and evaluate the results.Keep in touch.

**Update**: After I update the parameters for `basecall.py`, this is a new statistic result for the same UUID: ``` 5ec71e76-bd51-457d-b1f1-ad551ddae980 ACUACGAGGGUACCCGGGUAAUGCCGCAGGAAUUCUUUGGCCGCAGAACUUCCUUUCAAAGGCUAUUGCUACGGCGGAAAUUAGCUAAUGGUUACAGAAAGAAGCCCAUCCCCUCGGCCCGAAUAGUUUAUCGAAAUCGAGAAGGGCAGUGGAAAUUUCGACGGUCCUGACCGCAUCUCCGACUCCAUCCUUCCCCUGCACUCCUAACAGCAGCAUGUCCAAGAGCCCAUACCUGUCACUUUAGCCACUGGAGCUUCCACCGUCCCUGGCCUCUAAACCAUUGUAUCAUGGCUUCGCGACAAUUAAUGUGCCAAUACAUUCUUACGAGAGUUGGCCUUCAUUCUACCGAGUUUGAUAUGUACUUAUGCUGUUAGUGGUAGUUUAAAGCAUACCACCAAGGUACUGGCUUUGAUUUGUAUUAACCCUAGCAAGCCAUCUUAGCCAGGCUAUUGUGGAAAAGUCAACACCAUGAAGUUUGGAUGAUUGUUGCUGGAUCUUUUUCGGCUAAAUACAAGCUCCACGAAAUAGUCCGACGUCAGCCAAAUUCUUUGGAGCUAUCAAGGCUGCAAUGGCCUACUUCGCCUUAAUAAAACACCGACCAAUAUUUGAAAUCCCCCGUUGAAGCUGAAGUCAGGAUCAUUCCACUAUGCAAAACGUUGUUCCAUGAUCGACGCAGCACAAUUGGUAAUAGACGAGUCAAGGAUAUGUAAGUGGUAAAACUACGGCUGUGAUAUUCGCAUCGUGGACUUUGAGAAUCCCAAAGAAAAUGAGAAGAACCUUCUGCUCAUGGCGGUAAAGCCUAUGCAUGGCUAAGGCUGAUGACGAAAACUGAUGGUCAGUUGUUAUUUUGCCUCAGAAUGGUUGGUCACUAAUGCAGAAUGGAGCAACCUCGGCGGCUGUUAAAUCGUUUGCGCAUUGAUUCAUAUCACCACACUAUGGGUGACAUGUGGUGGCGAACUGUUAUGGUUUUUGUCCCCAGAGUUCUAAACAAGUCUAAUCGAAGAUAUGUUACGAAUCGUCUUUGGCAAAAAGAUUUUCGAGUGUCGAUUAAGCAGAUUCUCGUGGUCCAUUACUCCUUACUUUUUCCCAAGGUAUUGCGUCGCACUCGCGAUAACAUAAACCCUGAGAACAUUUUGAUCUCUUGGGUAUGGUGUUUUUAGAAUUCAUGGGGAUUUUUGGCGCUAUGGAUGUUUGUGCAAUCCCAGCGAUGAUAACUGUUGUGGAAUCAAAGAGUUAUGGGUAUUUUGGUGACCCCCCUCGGCUCCGUGAAAUUCGACUUCCAGGCUUUACAAUCCUUUUAUUGAUAUGACACACCUUAAUUAUCGAAUUCUACAUUGUCAUAUUUUUCGCCACGUUUCCCUUCCGCUAAAGCUUUUUCACGAGAACACCAGUUGGUACGCAAAAUUGUAAAGCUUGGAGAGAGUUUACAAUCUCAUUUGUGCACGUCCAAAAUAUUCGUAUUAAGAUCGCCAUUUUAAACCUGAUCGCAAUGACCUUUUUCCGAAUGCAGCAGGUUGUCUUGUCAUGGCAAAUCCCCAUUGCCAAACAAAGAAUUACUGCCCCCAAGAGAAGCUUUAAUCUCCCUUUGUCCAAGAAAUUGGCGCUGCUUUGUCGAUGAUUGGUUUAUGAUAUCCAGAGUGCCUUGAAACGAGCCAAAUCCUCCCGCUUCAGAGGACUAUCUACGCCACAAUUCAUGUUGAGAUUUUCGUAAUGGCUUGCUUCUUUGUUUUGAAGACGAUUAGUGUUUCAGAUUUUUUUUUAAUUAGCCAUUUUCUUGGUUGUUUGAAUUCAUCAACUCCGCUGCGCAUACUCCUCUCUUUUACCUUAUACUAUUAAUUUUACGACCGAAUUUCGAAAUGAUGUGUGUGUUUUGGCUGCCCCUUCGAAUGGUUACACUUCGACCCGAAGCUAUUUUAUUUGGAACAUGCAAGGCCCGAAAAAAUAAAAACGUCCAAGUUGGACUGACUUGCAAAUUAUAUCUACAAAAAGAAGAUGCCGCAUUGUUUUUUGUAUUCGAAUUUAGUUGAUGUAAUUUUUUAAUUUUUUAAAAUUCCAUCUUCCAUAAUUGCUACUUAUGCUUAAAUUUGGGACAAGCUACGUAUUGUAACGGCAUAGUGAGGAAAAUGGCCUUGUUUAAUAAUUUUUUCAGAGCAUUUUUUAUCAUAUUUGUAAUAAAGUCAGUAACGGCUUCGACUAAUCUACUGUUUGCGCUUACCUUAAAUAU ACCAAGTCTCCATGGTAATGCCAAGAATTTTCTTGATCGCAGAACTTCCTTCAAGGCTAATTACATGGCGGAAATTAATCAATGGTTACAGAAAGAAGCCCTCTCCCTTGCCCGAATAGTTTATTGCTGAGAAGGCGGTAATGGAAGCTATTCGACGGTCCATTGACCGCATCTCCGACTCCTCCCTGCACTCCTACTGCATGTCCAAGAGCCCATTCTGTCATTTAGCCACTACTGGAGCTTCCTACCGTCCTGGCCTTCTAAACATTAGTATCGTGTTCGCGACAATTATGCCAATCATCACACGAGAGTTGGCCATTCACTACCGAGTTTGATATGTTTACCATGCTGTTAGTGGTAGTTTAAGCTCTCCTCCAAGGTACTGGCTTTTGATTGTATTAACCCTGCAAATCCATTTAGCCAGGCTATTGGAAAGTCATCCATGAAGTTTGGATGATAATGTTGGATTTCGGCTAATACAAGCTCCAAATAGTCCGACGTCAGCCAAATATTTTGAGGCATCCAAGGCTGCAATGGCCCTACTACTTATCCTATAAAACACCGACCAATATTTCTGAATCCCCGCAGCTGAAGTCAGGATTTATTCCATATGCAAAACGCGTTGTTCCTCGATTGAGCGTAGAATCAATTGGTTGATGAGACCAAGGATATGTAGTGGTGCTACGGCTGTGATACGTTTGGACTCTTGAGAATCCCAAAGAATGAGAAGAACCTTCGCTTTGCGGTAAAGCCTATCGTCGTTAAGGCTGATGACGAAACTGATGGTCAGTATTGCTAAACTTGCCTCCAGAATGGTTGGTTCAATGCAGAATGGAGCATCCTAATGTTGTTAAATCGTTGATTTGTGCATTGATTCTCATTATCTTCCTCTCTATAGTGACACGTGGTGCGCTGTTATGGTTCGTCCCAGAGGGATCTGCTAAGTCTAATCGAAGATATGACACGATCGTCTTGGCAAAAAGATTTCGAGTGTCGATTAAGCAGATTTCGTGGTCTCATTACTCCACATTCCCAAGGTATTGTGCGCATTCGCGATATTAAACCTGAGAACATTTTGATTTAAGTATGGTGTTTTAGAATTACTGATTCTTGGCGCTGTGATGTTTTGTGCAATCCCGGTGACGATATTACTGTTGTGGAATCAAAGAGTATGGGTATTTGTAGTGACCCCTTCGGCTCCTGAATTTGACAAGGCTCTTACAATGCCTTTTTGCTGATATGTGATCTACCGCTATCGATTTTACATTGTCTATATTTCGTCATACCTTTCCGCAAAGCTTTCAGAACGACCAGTTGTACGCAATTGTAAAGCTTGGAGAGTACAATCTCATTTGCGATGTCCAAAATATTCGTATATCAAAGACTTGCCATATTTTAAACCAGTTTATAATGACCTTTCCGATGCTATGCAGCAGTTGTTTCTGTTTGGCAAATCCCATTGCCGAACAAAGAATTACTGCCCAAGAAGCTTTAATCTTCCCTTTGTCCAAGAAATTGAATGCTGTTTTGTCGTCGATTGTACACTATGATGCTCCAGAGGTGCCTTGAATGGGCAAATCCTCCTCGCTTCAGAAGTTATCTACGCCACAATCACTCGTTGAGATTTCGTAATGGCTTGCTTTGTTTGAAGACGATTAGTGTACGAGATTTTTAATAGCCATTTCTTGGTTGTTTGATTTTTATCATCAATGCCACGTTGTGTGCATACTCCTCTCTTCTCTCCTTATACTAACTAATTTCATGTACGATTTTCGAAATGATGTGTGTTTTGTTGCCCTTTGCTGGTTACACTTTCGACCTAAGCTATTTATTTGGAACCAAGGCCAAAAAAATAAAAACGTCCAAAGTTGACTGACTTAATAAATTATATACAAAAAGAAGATGCCGCATTGTTTTGTATTTGCTTAGTTGATGTAATTTTTTAATTCTTTAAATTCCATTTCCATTGCTGCATTTATGCTTAGCTACTAGTCAAGCTACGCAATATATGAAAATGCATAGTGAGGAATGGCCTTGTTTAATAATTTCAGAGCATTTTTATCATATTGTAATAAAAGTTCAGTAACGGCTTCGACTGCTTACTGTTTAGCGA 84.92208982584785 59.30366612866036 NM_001023527.2 2894 5076 ('NM_001023527.2',) 2849 5063...

@marcus1487 Thank you for your advice!I'll read the megalodon docs later.