vetmohit89

Thank you so much for prompt response. But I am not sure if we are giving all fast5 files as input file or individual file to run squigglePlot. I have...

Thank you so much for your help. It worked.

Hello, I am having similar issue in running tombo requiggle and tombo preprocess. Here are the steps I followed: 1. compress_fast5 -i ../fast5shGFP/ -s ../fast5_shGFP1_gzip -c gzip 2. Then I...

Thank you so much for comment. I have re-basecall fast5 files using following guppy commands guppy_basecaller \ -i /data/user/mbansal/Fast5/GFP/fast5_pass/fast5shGFP \ -s /data/user/mbansal/Fast5/GFP/fast5_pass/shGFP_FASTQ_2 \ -c rna_r9.4.1_70bps_hac.cfg \ --reverse_sequence \ --post_out \...

I don't think --fast5_out made any changes in original input fast files and also did not provide new fast5 files. Upon checking orignal fast5 files using h5dump -n Here is...

Hello Yuk Kei, can you please comment on it? I think @zzf77 has valid point.

I tried above suggested method to re-order the columns but no help. ``` Heatmap(Actinobacteria_counts_scaled_matrix_clean_sorted, cluster_columns = dend2, column_split = factor(as.character(meta_df$Species), levels=c('WT','db/db', 'db+ACE2', 'db+IW')), + column_order=column_order, + cluster_row_slices = TRUE, +...