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DIA-NN - a universal automated software suite for DIA proteomics data analysis.

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Hi, When i accidentally put variable modifications to Ac(N-term) and put on double-pass-mode, it says that it can’t run double-pass-mode so it is switched off. Does that mean, that single-pass-mode...

Hi, Is there a way to output all peptides with the chromatogram dataset? On the other hand, I was looking at the peak width (RT.Stop - RT.Start) in my data...

Hi Vadim, I recently forgot to destagger my raw files during mzml conversion. I fixed this now and both results seem to be very comparable when it comes to IDs....

Hi, I have generated a DDA spectral library following the DIA-SpecLib_Quant workflow. I am trying to quantify the DIA files in a separate run by providing the library.tsv file ,...

Dear authors, It is really a nice software. I want to use my MacBook to do the analysis in terminal. Is there any codes for this? Thanks

I am confused for the threads here and log level. Does it help to speed up. Or the Speed and ram usage of Ultra-fast is to help us speed up...

Hi, Vadim I recently want to test DIA-NN function of phosphosite localization. I downloaded the raw MS data which included 166 synthetic phosphopeptides spiked in yeast lysate and the spectral...

Hi Vadim, As always, thank you for developing this excellent tool. We performed library-free mode search against a FASTA file and in the identified peptides, we found a few do...

Hello, My question is in the title. Here are more details: I generated a spectral library with Fragpipe using unspecific digestion (immunopeptidomics data). I then would like to match this...

I ran two searches in parallel, one with .d TimsTOF Pro raw files one with .dia version of the raw files - to compare the speed gain. The search with...