DiaNN
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vdemichev
suddenly .d.dia raw files not loadable any more
I ran two searches in parallel, one with .d TimsTOF Pro raw files one with .dia version of the raw files - to compare the speed gain.
The search with the .d raw files ran through. The search with .dia raw files started normally and at some point all following .dia files could not be loaded, were skipped with 0 peptides/proteins identified. Subsequently, there were not enough peptides for normalisation. It appears unlikely that a network issue was responsible for that, because the other search with .d raw files did not have any issues. DA01.14_PL01_DA-SER1.15_dia files_.log.txt
Yes, if files are on a network drive, if network is unstable, errors can happen.
Well, as said, it appeared less likely that the cause was a network issue, because the other search using .d raw files did not have any issues.
Q1: Anyway, do I understand correctly that you don't see a reason that .d.dia files can make the search less stable? Q2: And that you think that a network issue still appears the most likely cause for you?
Yes, there are no known bugs, so if something is not working, it's probably the problem with sth else, not DIA-NN
Q3: In #413 you mentioned that you would not recommend using .d.dia instead of .d - as .d is relatively well compressed anyway. Is there a disadvantage of using .d.dia (information loss?)?
Second, is there still a search speed gain when using .d.dia compared to normal .d? (Or is it marginal?)
The only disadvantage of .d.dia is space on disk. Yes, there's still a speed gain.