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DIA-NN - a universal automated software suite for DIA proteomics data analysis.

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Hi, Excuse me again, for the Fragment.Info column in report.csv, does the combination of fragment ion type and loss (e.g. y10-H2O^2/563.2714844) only include the listed types, because the output I...

Hey, I found that the number of **Proteins.Identified** in the *rep.stats.tsv* file could not match the number of proteins in other matrix tables. The number of Proteins Identified in the...

Dear Vadim When I run one.mzML file on linux, I have no problem. When I run multiple.mZML files, I report an error: ubuntu@node188:~$ ./1/diann-linux --f data2/1.mzML --f data2/2.mzML --fasta data2/4.fasta...

Hello, Thank you for your great contribution. Will diann's output include the collision energy of precursor ions?

When using DIA-NN to analyze multiple DIA mass spectrometry data simultaneously, how should the parameters be configured for data normalization?

I used non-specific enzyme cleavage (Thermolysin), and there are no options available under the protease settings. How should I set the parameters?

Dear Vadim, I'm seeking clarification on the calculated scores for potential elution peaks. Could you explain the meaning of the 'pMs1Ratio' parameter? I noticed it doesn't appear among the 73...

Hello, I want to extract the 1/k0 values for my precursors from my DIA-PASEF run. In the DIA-NN output there are two ion mobility entries: IM and iIM that are...

Dear Vadim I performed a DiaNN analysis on my MDA-MB-231 vesicular protein samples. However, at the end of the identification process, my protein group file was empty, even though I...

Working to parallelize my pipeline, based on the following: _"Another option is to reduce the precursor mass range, that is search mass ranges 400-500, 500-600, 600-700, etc, separately - create...