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Published 20 hours ago • verified publisher icon vdemichev

data normalization

Open fei2805 opened this issue 1 year ago • 4 comments

When using DIA-NN to analyze multiple DIA mass spectrometry data simultaneously, how should the parameters be configured for data normalization?

fei2805 avatar Dec 14 '23 01:12 fei2805

Data normalisation is automatic, so please just follow the guidelines here https://github.com/vdemichev/DiaNN?tab=readme-ov-file#getting-started

vdemichev avatar Dec 14 '23 10:12 vdemichev

Thank you for your response. Is the intensity provided in the report (eg. report.pr_matrix.tsv, report.pg_matrix.tsv) LFQ intensity, and can it be directly compared quantitatively? When conducting differential analysis (upregulation/downregulation), is there a need for additional correction of the intensity results?

fei2805 avatar Dec 15 '23 07:12 fei2805

Can use as is

vdemichev avatar Dec 15 '23 22:12 vdemichev

Can use as is

Thanks a lot！

fei2805 avatar Dec 17 '23 13:12 fei2805