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DIA-NN - a universal automated software suite for DIA proteomics data analysis.

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Dear Vadim, Can DIANN1.8 use metageomic protein fasta to predict spectral library? Best, Yingying

Dear Vadim, We have been using DIA-NN for a while and it is great. Recently we have experienced some issues when trying to analyse TIMS-TOF DIA-PASEF data from plasma samples...

Hi. I was interested in understanding in more detail how DIA-NN implements the MaxLFQ algorithm and if it is possible to specify certain parameters in the command box. I understand...

Hi, I'm new to DIA-NN and interested in analysis of DIA-FAIMS files. Basically each sample has been run with 4 different CVs , So we have 4 files per each...

Hello Vadim, I ask myself how to process different sample runs in DIA to generate a huge sample specific library. I measured different sample types in gas-phase fractionation mode and...

Hi, I need decoys to run the protein quantification tool Triqler. Is there a way to get DIA-NN to output the decoy peptides? Best, Patrick

Hi, I am testing the library-free search using DiaNN. I ran DiaNN in Windows and Linux with the same input file, fasta file and parameter setting. I found that there...

Hi, Thank you for the great tool! When running the same analysis one two different computers the number of identified proteins changed a lot. This trend was observed repeatedly, one...

Hi, Vadim Thanks for your work of DiaNN. When I use DiaNN in library free mode in command line on my linux( ubuntu) system, I got an error showing 'Segmentation...

It appears that lower limit on peptide length is ignored - seems to be set at 7 regardless of value set (log reports change). Thanks