Umer
Umer
if someone is facing this issue now, then try replacing /usr/local/lib/python3.6/site-packages/grabseqslib/sra.py **line 45** with metadata = requests.get("[https://trace.ncbi.nlm.nih.gov/Traces/sra-db-be/sra-db-be.cgi?rettype=runinfo&term="+pacc](https://trace.ncbi.nlm.nih.gov/Traces/sra-db-be/sra-db-be.cgi?rettype=runinfo&term=%22+pacc))
Dear Adam, Thank you for your detailed responce. It clerifies alot of things.
@zhaolei6116 Thank you for explaining.
Hi @Adamtaranto , Thank you for detailed responce. To answer some of your questions. "**How complete is your current assembly - near chromosome level?**" my assemblies are near to chromosome...
Hi, Nice guess. These are indeed Fusarium spp. genomes. I assembled them using `hifiasm` and removed duplicate haplotigs using `Purge_Dups` ( i guess that is where sub-telomeric regions were lost)...
For me, the **median read length:** ~13.5 kb to ~18.1 kb **read length stdev:** ~4,700 to ~6,800