ramnageena11

Can't locate Bio/Root/Version.pm in @INC (you may need to install the Bio::Root::Version module)

19

powersequence@CH-CB-POWERSEQ:~$ prokka --help Can't locate Bio/Root/Version.pm in @INC (you may need to install the Bio::Root::Version module) (@INC contains: /home/powersequence/anaconda3/bin/../perl5 /home/powersequence/anaconda3/lib/site_perl/5.26.2/x86_64-linux-thread-multi /home/powersequence/anaconda3/lib/site_perl/5.26.2 /home/powersequence/anaconda3/lib/5.26.2/x86_64-linux-thread-multi /home/powersequence/anaconda3/lib/5.26.2 .) at /home/powersequence/anaconda3/bin/prokka line 32. BEGIN failed--compilation...

Command: fastp -i /media/majorram/Analysis_Data/singhrn/ramesh/P1_S23_R1_001.fastq -o /media/majorram/Analysis_Data/singhrn/ramesh/fastp_qc/fastp_P1S23_R1 -I /media/majorram/Analysis_Data/singhrn/ramesh/P1_S23_R2_001.fastq -O /media/majorram/Analysis_Data/singhrn/ramesh/fastp_qc/fastp_P1S23_R2 --unpaired1 /media/majorram/Analysis_Data/singhrn/ramesh/fastp_qc/unpaired1_P1S23 --unpaired2 /media/majorram/Analysis_Data/singhrn/ramesh/fastp_qc/unpaired2_P1S23 --overlapped_out /media/majorram/Analysis_Data/singhrn/ramesh/fastp_qc/overlapped_P1S23 --failed_out /media/majorram/Analysis_Data/singhrn/ramesh/fastp_qc/failed_P1_S23 -m --merged_out /media/majorram/Analysis_Data/singhrn/ramesh/fastp_qc/merged_P1S23 --thread=10 terminate called recursively terminate called recursively terminate called...

FileNotFoundError: [Errno 2] No such file or directory: '/home/majorram/anaconda3/envs/checkm/hmms/phylo.hmm'

2

Hi, I am geeting this error. Pls suggest. checkm lineage_wf /media/majorram/Analysis_Data/singhrn/medaka_final2/s2mags_genomes/s2mags_contigs_16S/ /media/majorram/Analysis_Data/singhrn/checkM_metagenome/S2_metaAssmbly/S2mags/ --reduced_tree -x fasta -t 32 [2023-08-02 12:16:36] INFO: CheckM v1.2.0 [2023-08-02 12:16:36] INFO: checkm lineage_wf /media/majorram/Analysis_Data/singhrn/medaka_final2/s2mags_genomes/s2mags_contigs_16S/ /media/majorram/Analysis_Data/singhrn/checkM_metagenome/S2_metaAssmbly/S2mags/ --reduced_tree...

Memory requirement and path for database to run metagenome-atlas

5

Hi, I have 70 Gb shotgun illumina PE data from 4 samples with 3 replicates. I need your help regarding Running of metagenome-atlas for analysis: How much RAM do I...

Hi I'm running medaka consensus for a metagenome assembly from nanopore reads. but it giving the error of "-d must be specified". I have tried to run the command from...