qingbo11

Hi Dr. Torsten Seemann, I addressed this issue as you mentioned in the previous reply, I changed the folder and found the ref seq file, then it works well. Thanks,...

Hi Dr. Seemann, When I use the snippy-core function, and add —mask Mtb_NC… parameter, it showed an error as below. I used the same ref seq in .gbk format for...

Please try this "snippy-core --ref YOUR REF SEQ FILE.gbk --prefix TB0065_S16_L001 TB0207_S6_L001"

Everyone who knows this better please correct me if I am wrong. My understanding is that the ref seq is used to align all seqs including your out group one....