Jon Palmer

Hopefully that error makes sense - you have sequences (at least one) that doesn’t have any taxonomy information.

UTAX is specific to usearch. The memory limit doesn’t seem to always be consistent, technically it should be 4 GB but that doesn’t mean it can work on files equal...

Malloc errors are memory related, so the free version of usearch is 32 but and thus the file you are trying to make a database out of is too large...

It looks like the ITS database is either corrupt or not installed properly. What is the output of `amptk info`? ie for me: ``` $ amptk info ------------------------------ Running AMPtk...

Sorry I didn't notice the first issue -- the formatting was all blended together. Can you rephrase the first issue and include commands you used to generate those errors?

its probably just a pandas issue, try to downgrade to something less than v2.0 and see if that works, ie `python -m pip install "pandas

I was thinking same thing when I saw your tweet this morning. Probably a few mods and it should work, but right out of the box I'm less sure of...

In the past I've used PoreChop https://github.com/rrwick/Porechop for demuxing and adapter trimming (Ryan writes really nice tools). I think I would not quality trim the data at all actually, it...

If I remember correctly, both PoreChop and now Albacore demux files into separate folders. So something like how `amptk illumina` should be somewhat easy to write, basically lift the sample...

Actually I think this already exists, the `amptk SRA` demuxing script takes a folder as input and fastq files in that folder get processed, i.e. the file name is used...