Jon Palmer
Jon Palmer
If you can let us know how to run it properly with the larger reference I can probably update the minimap2 command accordingly. Also would be helpful to understand the...
Hi @Cat4Lion, so it seems you are running a development version, ie 1.8.10 -- all of the even numbered are not releases but rather at an intermediate step. The error...
I can't say it was ever a problem. Funannotate just looks in the path for signalp, if it finds it then it tries to run it. If it doesn't than...
`--reference` does not fix the issue in v2.2, the reference alleles are still "N". This is incorrect (it seems) based on the VCF 4.2 spec. To fix seems like the...
lol. Amazing. Thanks for your continued efforts!
Thanks for question @mbcarbonetto. FYI, this was published several years ago here: https://peerj.com/articles/4925/. In the paper we did essentially two different things: 1) PCR amplified the product from each plasmid...
Yeah -- there is some basic/rudimentary processing in the codebase already -- so demultiplexing the reads is easy and already done. The most difficult part will be dealing with the...
I would be very surprised if it works in windows. It has never been tested on windows (at least by me) so likely there is a dependency or two that...
update: I've tried this, but the size of the database is large and searches are incredibly slow, need to figure out why before doing this. There seems to be a...
Likely running out of memory or the taxonomy labels not formatted correctly. Check the logfile to see what usearch died with.