Guanliang MENG

Hi Phismil, I had a look your log file, the related information is this: ``` 2023-09-15 12:00:41,584 - mitoz.utility.utility - INFO - rRNA_annotation() return: s_rRNA_ft: /mnt/lustre/users/aemami-khoyi/eDNA/map/tmp_SW_sw_map.fasta_mitoscaf.fa/SW_sw_map.fasta_mitoscaf.fa.s-rRNA.ft l_rRNA_ft: /mnt/lustre/users/aemami-khoyi/eDNA/map/tmp_SW_sw_map.fasta_mitoscaf.fa/SW_sw_map.fasta_mitoscaf.fa.l-rRNA.ft 2023-09-15 12:00:41,584...

Hey, Thanks for pointing out the problem! I will add the information in the next release. The HMM files are used to screen out candidate mitochondrial sequences initially, and the...

Hi Phismil, In MitoZ paper, >We further annotate the putative control region in the case that the remaining interval region has a length ≥ 600 bp ([48] and all its...

Thanks, @Phismil! It would be great if you can provide a few publications about "some might have more than one control region." I'm interested in having a look at them.

Hi Phismil , Thanks for it!!

Hi @kabilov , Thanks for reporting the issue! I tested your command ``` mitoz annotate --fastafile NC_012920.fa --genetic_code 2 --clade Chordata --thread_number 4 --outprefix test ``` and the problem can...

This command failed: ``` megahit --out-dir ./megahit_out --num-cpu-threads 16 --k-list 59,79,99 --memory 1500000000000.0 -1 /home/ubuntu/Idmi_reference/clean_data/Idmi.clean_R1.fq.gz -2 /home/ubuntu/Idmi_reference/clean_data/Idmi.clean_R2.fq.gz ``` perhaps your server ran out of memory?

I do not remember if the "megahit" directory has the log from the megahit command, you may check it first; and you should also check the stderr file from mitoz...

> yes i run the code and here the error  thanks for the post. The last line says that you are running out of disk space on your machine...

computer memory and disk space are different things. your current problem is there is not enough disk space on your server, which can not be solved by using the `-memory`...