learning-MD

No signaling genes when using mergeCellChat()

1

Hi, I've got a scRNA-seq dataset with 2 different conditions. I've split them into separate Seurat objects and made cellchat objects with them individually. I can run through the single-condition...

Hashed samples in 10X

2

Hi, first of all, thank you for this fantastic resource! I have 5' scRNA-seq data on PBMCs from 40 patients that have been hashed with antibodies - 5 samples tagged...

Unable to run via Singularity - any issues with Singularity image?

2

Hi, thanks for a great tool! I installed Singularity and confirmed it's working fine. However, whenever I run anything related to the following code: `singularity exec souporcell_latest.sif souporcell_pipeline.py` I just...

Hi, this looks to be a wonderful tool - thank you for making this for the community! Our group is working with single-nuclear samples from solid organs and we're interested...

Creating metadata file for multiple hashed samples

2

Hi, I have a multiplexed 10x 5' gene expression run (5 samples hashed together) and the TCR/BCR reconstructed using TRUST4. In such a case, where there are multiple samples all...

Issue with hashed samples and merging TRUST4 results with Seurat object

21

Hi, Thanks for this great tool and the great support you guys have provided here! I had previously posted about running TRUST4 on multiplexed 10X 5' human PBMC scRNA-seq samples...