LeeLee

> The problem has been solved and can be run according to this version of the software > > ``` > tophat2：tophat-2.0.12.Linux_x86_64---------------------http://ccb.jhu.edu/software/tophat/downloads/ > samtools：0.1.18.0------------------------- > bowtie：1.0.0.0--------------------------https://sourceforge.net/projects/bowtie-bio/files/bowtie/1.0.0/bowtie-1.0.0-linux-x86_64.zip/download > ``` Hi, zxclovezby...

Oh, it suddenly occurred to me that I could run the files in test_data, and I got the desired result. It seems that the software did not find circularRNA in...

> FragPipe/TMT-Integrator does not have the isotope impurity correction. We used to have it but removed because it was creating some new problems. When you have empty channels, annotate them...

The treated_array.txt file does not seem to be uploaded well, I will upload it again [treated_array.txt](https://github.com/YuLab-SMU/MicrobiotaProcess/files/11269290/treated_array.txt)

Yes, this greatly improves the speed of mp_rrarefy, but it seems to be still slow for mp_cal_rarecurve