lbleicher

We are trying to run Topiary with sequences that seem to have no paralogs in most clades, but that might be named differently according to the species. So our database,...

Error in topiary-seed-to-alignment

25

I attempted to create an alignment from a seed of six sequences from four species (this is my input csv file): =========================== species,name,aliases,sequence,accession Homo sapiens,TTHY_HUMAN,hTTR,GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE,P02766 Saccoglossus kowalevskii,D1LXG7,Acorn worm HIUase,MSGYRIDILTNHLRASQAHSNLIEAVNMAGQQSPLTTHVLDTALGRPAAELPITLYSRSPEMAWLKIAAGKTNQDGRCPGLLTQETFHNGVYKIHFDTGTYHKALDTPGFYPYVEVVFEIHDPNQHYHVPLLLSPFSYSTYRGS,D1LXG7 Danio...

All aditional sequences removed in seed-to-alignment step

8

Hi, I've tried to run seed-to-alignment in another computer and this time it ran to the finish, but all sequences were removed (they get flagged Keep=false) and the final file...