l.gallucci
l.gallucci
Hi! I have problems with installation related to channels, for tools like samtools or perl-dbd-mysql...it's assessing that this packages are not available in the channel...can you confirm that or in...
Hi there! I'm working with bacteria and archaea 16S (MiSeq sequencing). More in specific I'm dealing now with the Archaea one (V3-V5) region. I was reading different issues (both open...
Hi! I'm working on different samples which has been sequenced with a particular set of primer, so I'm running your protocols for ITS but with Bacterial 16s sequences. We notice...
Hi all, I'm trying to install a package which has a dependencies based on bedtools, the hardware is a Macbook pro. I read about people that got the installation through...
Hi! I'm trying to install Phyloflash on my macbook, but during the installation is not able to find the bedtools release in any of the conda channels. Suggestions?
Hi! I'm working on 16S sequences from Illumina 2x300, with targeting regions V3-V4 (341F-785R)... Forward: Reverse: These are the quality profile after cutadapt. We were trying to target the lenght...
Hi there! I'm following the guidelines for the mixed oriented reads case, trying to demultiplex a bunch of 4 different files (x_R1.fastq.gz, x_R2.fastq.gz, y_R1.fastq.gz and y_R2.fastq.gz). After running the pipeline...
Hi @benjjneb, I have several questions, hopefully not all stupid ones. I'm working with sequences with both a classical set of primers... ``` FWD
Hi all, I'm using the barcoding kit SQK-NBD114.24 for sequencing on MinIon Flow cell. Before the library preparation and sequencing, I'm doing on each samples a pcr with barcoded primers:...
Hi @marcelm, thank you for the development of Cutadapt. I'm actually using the latest stable version and python 3.10. I'm dealing with these heterogeneity spacers+primers: Bacterial region V3-V4: 341F (5´-CCTACGGGNGGCWGCAG-3´)...