l.gallucci
l.gallucci
@benjjneb I'm trying to install dada2 with last version of Bioconductor on my new mac, but it fail to complete the installation of different dependencies. ``` In file included from...
Someone has solved that problem?
To add additional context and an additional question: I'm using Cutadapt 4.5 and python 3.10 with the following script to demultiplex the files that I have: `/bioinf/home/lgallucc/software/miniconda3/envs/tagseq/bin/cutadapt \ -j 30...
> That said, you may want to instead update to Cutadapt 4.6 and use the --revcomp option: With it, only one round is needed. Cutadapt will swap R1 and R2...
Yes, the problem was this related to that command, but... the problem is that using `--revcomp` as option the command is working, but the output is not detailed, just: ```...
@benjjneb thank you for your reply! Yes, of course. So, improving `filterandTrim` giving a look to the quality Plot is your suggestion, as I understood well, right? About that, if...
Yes, this is what I'm finding after the merging. So, ideally, the solution will be to not giving so much importance at the percentage after the `filterandTrim` but rely more...
Hi @benjjneb, adjusting a little bit the code, and with the following setting: ``` out
Set for chimeric removal: `seqtab.nochim
I agree with that, so I changed the clipping values to `truncLen = c(280, 260)`. The problem at the base is that the reverse quality is really bad, coupled with...