Chris Seymour

Hi @gao9842 Can you try with `v0.5` and a single GPU?

Hey @cnk113 Yes, we take the reverse complement by default but as you have found it's an easy modification if you want to try the approach with multiple copies of...

@Saranya-nanobiophotonics-lab GTX 1050 is a pascal card and bonito depends on volta or above.

Hi @CDieterich I have not trained an RNA model yet, I will update this issue if things change. Regards

I think it should be straightforward, if not, let me know. First, make sure you have the training data downloaded. ``` $ bonito download --training ``` Then run bonito train...

@snower2010 `bonito download --training` will give you the full training set for `dna_r9.4.1` that is used to train the model shipped with bonito. I'm currently only focusing on a single...

Hey @89213385 1. You can use a specific set of reads as a validation ctc set for training by saving them inside the training directory in a `validation` folder (https://github.com/nanoporetech/bonito/blob/master/bonito/cli/train.py#L41)....

The `--ctc-min-coverage` checks the called sequence against the target sequence. Low coverage would mean samples in signal would not be assigned to any bases. https://github.com/nanoporetech/bonito/blob/503fa9a4ff445c40ecff2db7a0308ecc2838a77e/bonito/io.py#L414

Hi @yanchunzhang What is the typical read length in your dataset?

Hey @danrdanny Thanks for reporting - we will need some time to investigate this in detail.