poreplex
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A versatile sequenced read processor for nanopore direct RNA sequencing
Hi, Use of --barcoding and --fast5 give error. Here is the command and the error. Thanks poreplex -i INPUT -o OUTPUT --barcoding --trim-adapter --basecall --parallel 5 --fast5 Poreplex version 0.4.1...
Hi, I'm running poreplex in a linux machine using the following command: poreplex -i /home3/Data/Nanopore/DIRECT_RNA/run_250319_Wt/fast5_pass/ -o /home/epicardi/Nanopore/pass --trim-adapter --parallel 20 --align /home/epicardi/minimap2index/mm10.mmi --fastq For each running process I'm getting: 2019-04-02...
Hello, I'm a little confused about some of the info in the poreplex log file. I'm running poreplex with the --basecall flag, but in the log file I'm getting a...
Hi! Thanks for nice tool, we are using it now extensively. Can we expect integraton with latest albacore? And if so at what timeframe? Or is it possible to use...
I'm getting this error (see below). I used ont_fast5_api to demultiplex the multi-fast5s and then ran poreplex on those fast5s. Any input would be helpful. Thank you. 2019-01-31 14:21:01,777 Command...
Hey, see title. Also, is it not feasible to split the read und process/keep both fused reads? Cheers