error when barcoding and fast5 flags used

[teshomeb]

Hi, Use of --barcoding and --fast5 give error. Here is the command and the error. Thanks poreplex -i INPUT -o OUTPUT --barcoding --trim-adapter --basecall --parallel 5 --fast5

Poreplex version 0.4.1 by Hyeshik Chang [email protected]

• Cuts nanopore direct RNA sequencing data into bite-size pieces for RNA Biology

== Analysis settings ======================================

• Input: INPUT
• Output: OUTPUT
• Processes: 5
• Presets: rna-r941.cfg
• Basecall on-the-fly: Yes (albacore 2.3.1)
• Trim 3' adapter: Yes
• Filter concatenated read: No
• Separate by barcode: Yes
• Real-time alignment: No
• FASTQ in output: Yes
• FAST5 in output: Yes
• Basecall table in output: No ===========================================================

==> Processing FAST5 files ERROR: Unhandled error [Errno 2] No such file or directory: '~/fast5/pass/BC3/FAK84611_6b938d7e6efe081830fee799484ebdfbbf1725f6_3.fast5'Task exception was never retrieved future: <Task finished coro=<ProcessingSession.run_process_batch() done, defined at ~/lib/python3.5/site-packages/poreplex/pipeline.py:183> exception=CancelledError()> concurrent.futures._base.CancelledError | 100% of 665704 |################################| Elapsed: 0:00:19 Time: 0:00:19

==> Terminated.