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huangyh09
BRIE: Bayesian Regression for Isoform Estimate in Single Cells
Hi, I run brie-count on a .bam file and use the provided SE.gold.gtf annotation. Unfortunately, I receive this error message:  What is the reason...
Dear Authors, I have sequenced, let's say, 1000 cells. I run brie2 in two ways: Strategy1, run brie2-quant for 1000 cells; Strategy2, run brie2-quant for 1-500 cells and for 501-1000...
Hi,thank you for developing BRIE! I've processed all my data with Brie2, but I'm wondering how to look at specific sites where differential splicing occurs, such as which exon occurs,...
Hi together, When I count MXE events and run brie quant on it, I get a file where the chromosmecoordinates are listed (format: chr6:10749202:10749268:+@chr6:10749622:10749698:+@chr6:10755142:10755232:+@chr6:10770077:10770204:+ 24905) and p values etc. But...
Hi @huangyh09, thanks for this amazing tool! I n the outputfile (clusters.tsv), I can see two columns for p_values. One is called p_value and one is called condition_pval. What is...
Dear author, 1. I think the psi 95CI shuld be a invterval. but only a single value was provied in the result. Is Psi_95CI the upper bounds of 95 CI?...
Hi Yuanhua, Thanks for developing this amazing tool! I'm interested in detecting AS events in my paired-end smart-seq2 dataset and when I was running the brie-count function: brie-count -S cell_table_smartseq.tsv...
Hello!Thank you for developing Brie! I am interested in alternative splicing events, so I applied BRIE2 to my research. I have eight 10x genomics samples which belong to four groups....
Dear Yuanhua, Thank you for developing BRIE! Now I have eight samples which have sequenced by 10x genomics, so I get 8 bamfiles and 8 barcodes.tsv.gz. In your introduction for...
Hello!Thank you for developing Brie! Recently I tried to visualize sashimi plot with Brie2's h5ad files. However,I noticed that I had to use Brie1's results(samples.csv.gz)to draw the sashimi plot with...