Francisco Rodríguez-Algarra

The *.prev file does seem to be truncated ``` $ tail -n 3 [...]/sequencing_summary.txt.prev PAG50866_pass_a8c5e533_15.fast5 2a66c331-32e9-41c2-b386-eb15743b4918 a8c5e5330539ae4e22df7db35b72c833dbb28282 91 517 2 8131.663000 79.819000 23945 TRUE 8134.889000 22977 76.593000 3394 9.965094 0.000000...

For the PromethION run: ``` $ head -n 5 [...]/sequencing_summary.txt filename read_id run_id batch_id channel mux start_time duration num_events passes_filtering template_start num_events_template template_duration sequence_length_template mean_qscore_template strand_score_template median_template mad_template scaling_median_template scaling_mad_template...

Thanks, Jared. So if the file that was actually used appeared correct, do you have any suggestion of why would it have crashed? Any other test you want me to...

Thanks, Marcus. It would indeed be really really useful if this could be incorporated into future releases.

Asking my experimental colleagues, I just realised that the 13 bp indicated in the text are (as the document explicitly mentions) only the start of the adapter sequences, which are...

Thanks so much, Felix. It is much clearer now. Nevertheless, as I mentioned before, another recent sequencing run of ours shows median insert sizes even lower than any other RRBS...

Hi @FelixKrueger , I have done some further QCs, and it seems to me that this issue is more generally concerning than I first anticipated. I thought this would only...

Thanks, Felix. I guess being purely computational myself, I find it hard to wrap my head around the idea of insert sizes being truly so small. I assumed size selection...

Just to clarify, all libraries in each figure have been generated using exactly the same procedure, and yet their (median) insert sizes are somewhat different. We are not comparing different...

Now that we know of this potential impact, we will make sure to not rely on the overall values. But perhaps it's worth stating it clearly in the documentation? I've...