frankbioinfo

Sorry all, i was able to install it using docker image, can i request if anyone from the team can build a docker image for this it will be very...

[PrimerDesign 2.cfg.zip](https://github.com/zichner/primerDesign/files/1010670/PrimerDesign.2.cfg.zip) [exampleVar (1).txt](https://github.com/zichner/primerDesign/files/1010671/exampleVar.1.txt) command: python primerDesign.py PrimerDesign.2.cfg exampleVar.txt results.txt

does it help?

No error related to primer3. but here is weird thing I get an output file but still see these error (attached result out file and the full error file. (can...

Ah ha!, the docker to rescue again! thank you docker should solve most of the issues. will keep you posted.

@zichner is this correct? using stringent25bp and example file as is please see below log and error docker pull zichner/primer-design Using default tag: latest latest: Pulling from zichner/primer-design b6f892c0043b: Pull...

Docker run with multiple options #1 docker run zichner/primer-design python primerDesign.py 2017-05-31 16:16:44.930912 Traceback (most recent call last): File "/opt/primerDesign/primerDesign.py", line 471, in main() File "/opt/primerDesign/primerDesign.py", line 24, in main...

[results.txt](https://github.com/zichner/primerDesign/files/1043052/results.txt) Option1 worked! Thanks attached result and log, any red flags? [primerdesign.log.txt](https://github.com/zichner/primerDesign/files/1043053/primerdesign.log.txt)

Thank you for your help, I have one last question what was your approach to build the example variant file do you have a snippet of the input and output...