Daniel Straub

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If I was too quick with closing that issue @tjcreedy (i.e. because it wasnt solved completely), please open it again!

Phylogenetic placement is now implemented in [nf-core subworkflow `fasta_newick_epang_gappa`](https://github.com/nf-core/modules/pull/2856) and in https://nf-co.re/phyloplace. Would you like to attempt to integrate it into ampliseq?

This issue was broken into https://github.com/nf-core/ampliseq/issues/561 & https://github.com/nf-core/ampliseq/issues/562, so I close this one here.

That seems to be completed by #518 and #534 ?

Then I'll close it for now.

What about adding a few optional columns (such as fw_index, rv_index) to the sample sheet. If those columns are present, demultiplexing will run. If that might mess too much with...

You could also check out https://nf-co.re/demultiplex (that I have never used) to apply first and then use ampliseq. If you do, let us know if that works as expected. Just...