Christophe Leterrier

I also have never managed to get the "Restrict ROI" working after importing localization files.

Dark offset has to be entered in ADU i.e. 100 Christophe Le mer. 2 août 2023, 10:24, Wohl Cellular Imaging Centre < ***@***.***> a écrit : > Wondering if there...

Hi, **Sigma** for a localization is the width if the actual spot in the image in nanometers. It is usually around 160-200 nm for STORM with Alexa Fluor 647. **BGSTD**...

Hi Anne, Happy to help! Actually I'm not an author of ThunderSTORM, more a regular (and happy) user. I made scripts to translate localizations file to and from ThunderSTORM format,...

Just checking what is the current state of this? Due to problems in building from source, I'm using the one-click installer. I tried to add the current GPUfit python packages...

I have found that there is a minimum value for the smoothing factor below which there will be an error. It depends on the number of substacks (i.e. more substack...

When I plot the coordinates obtained using DECODE in another software (exporting as csv and using ThunderSTORM in ImageJ), the image is rotated compared to processing by other softwares. I...

See also the Z axis being reversed (opened a Discussion about it)