Base level offset

[davepypy]

Hi,

we're using a Hamamatsu ORCA Flash 4.0 V2 sCMOS camera for which the stated e-/ADU conversion factor is 0.46 e-/count and "dark offset" is 100 counts:

However, if these values are entered into the camera parameter settings, ThunderSTORM complains that the base level offset is higher than the signal:

What might be the issue here? Is it correct that the "dark offset" has to be given in ADU counts and not electrons?

Thanks and best regards, Dave

[kclwcic]

Wondering if there was a resolution to this - Dave, did you find the answer?

[cleterrier]

Dark offset has to be entered in ADU i.e. 100

Christophe

Le mer. 2 août 2023, 10:24, Wohl Cellular Imaging Centre < @.***> a écrit :

Wondering if there was a resolution to this - Dave, did you find the answer?

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