biochem_fan

At least `FileName::validateCharactersStrict()` already rejects such names, because it does not accept `_`. However, this does not guard from file names like `Micrographs/data_set_0000.tif`.

https://www3.mrc-lmb.cam.ac.uk/relion//index.php?title=FAQs#Refinement_or_Classification_crash_saying_.22No_orientation_was_found.22

You are selecting a wrong STAR file. You should select a particle STAR file from an Extract (or Subset selection) job, not AutoPick coordinate STAR files.

What happens if you ignore CTF until first peak, or completely disable CTF correction? What if you set `--maxsig 100`?

Thanks for your bug report. Until we fix it, please run with very tiny sigma offset and sigma angles (e.g. 0.01), which are effectively zero.

I cannot reproduce your problem. Can you show me your body STAR file? Does this happen in the first iteration, or later?

Does `Combine iterations through disc?: Yes` in the `Compute` tab help?

## MPI error Because I cannot reproduce your issue, I cannot help further. Recompiling with a newer version of OpenMPI might help. ## No convergence Look at these lines in...

But computationally more demanding.

> -Doing a new initial model (this works ok) and then using this model for a refinement (gives same error) Are you sure your mask and the new initial model...