biazen123

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PerMANOVA for trans_alpha$cal_diff()

Hi Chi Thank you very much for your prompt suggestions.

Allowing "justconcatenate = TRUE" to minimize loss of much reads during merging stage

Hello @benjjneb Thank you very much for your technical services and suggestions.

Allowing "justconcatenate = TRUE" to minimize loss of much reads during merging stage

> The sequenced amplicon you are working with is only 251-256 nts, so there is not really any meaningful gain in length of merged amplicon from joining forward/reverse reads here,...

Allowing "justconcatenate = TRUE" to minimize loss of much reads during merging stage

> The length of the V4 amplicon using the most common primers for this region is 251-256 nts. > > The most common V4 primer sets do not sequence the...

Allowing "justconcatenate = TRUE" to minimize loss of much reads during merging stage

> Yes, use `truncLen` instead of `minLen`. Thank you very much for your technical support. I will proceed based on the suggestion.

relative abundance for dominant functions for 16S using FAPROTAX

Hi Thank you very much for the clarity. Best.

relative abundance for dominant functions for 16S using FAPROTAX

> Hi. If you run `colnames(fun_16S$res_spe_func)` or `colnames(fun_16S$res_spe_func_perc)`, you can see there are three similar names: "aerobic_chemoheterotrophy", "chemoheterotrophy" and "anaerobic_chemoheterotrophy". Actually, the initial FAPROTAX database matching result has no "anaerobic_chemoheterotrophy",...