dada2
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Accurate sample inference from amplicon data with single nucleotide resolution
Hello, while running a lot of different projects with some project downloaded from SRA having more than 3000 fastq files I realised that mismatch files can occurs quite often. It...
Hello, I am having an issue with the filter and trim package Thanks for any help! I've been looking through the forums with no luck so far. These are the...
### Overview We are encountering some issues in obtaining appropriate estimates of error rates; I believe these problems are coming from our new(-er) sequencing facility sending us fastq files containing...
I'm working with a environmental dataset from a 3 year sampling campaign in a freshwater lake. I sequenced one marker gene from 215 samples and split randomly the sequencing of...
Hi all, I am analyzing data from fecal sequencing of 16S rRNA V1-V2, primers: 27F and 338R (MiSeq 2x300 bp). According to quality graphs, the sequencing didn't go well, especially...
Dear dada2 users, I am trying to use our customised data set. Genus format is working for species fpormat is not working I tried a lot but i could not...
Hi! First of all, thank you for this fantastic tool and the support here on GitHub. I am working with an ITS sequencing from tropical soil samples, and I have...
Dear developer, I am processing Illumina 2x250 paired-end reads corresponding to a 16S V3-V4 region sequencing. My usual pipeline implies primer and adapter elimination with Cutadapt followed by quality trimming...
Hello! I was analysing 16S NGS data for a study that will correlate culture x 16S NGS results: On the first analysis, I kept the 'minoverlap' default and we had...
Hy! I'm working with ITS sequencing data processing in DADA2. However, I have no information on which set of primers the previous researcher used for sequencing. Is there any program...
