Anton Korobeynikov

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You SPAdes job was killed from outside. You may want to check your system log for more information (or ask your system administrator)

SPAdes 3.13.0 you are using is ancient. Please try the latest SPAdes 4.0

> May I ask how to update to spades 4.0 on hpc ;-; Conda doesnt work :( and I also tried to drag the downloaded packet to my hpc but...

I would suggest you to ask your system administrator. This looks like some problem at your end, e.g. some i/o problem

Likely there is some filtering in the middle. In any case, the binaries are available on github as well

All releases are on github. Take 4.0.0 from https://github.com/ablab/spades/releases/tag/v4.0.0

Hello There is an I/O problem. SPAdes was unable to read one of its intermediate files. So, maybe more information is available in the system log? Does moving output /...

Does it reproduce with another dataset? How about running `spades.py --test`?

> Without specifying the threads option, it runs smoothly, but of course it would be good if I could use all or most of the cores of the server... You...