Anbu Poosakkannu

It worked for me. Thanks.

dataset.rdata.zip pls find the dataset attached! On Thu, Feb 2, 2023 at 3:48 AM Chi Liu ***@***.***> wrote: > Hi. Could you please send me your dataset2 to check it?...

Thanks, check the following link, https://drive.google.com/file/d/1QI_OcO4Q8TtJXMrQaSEiVhutseYKfaW3/view?usp=drive_web

Thanks, it worked. It seems that when I do the subset, a factor still remains even after tidying the dataset. On Tue, Feb 7, 2023 at 10:56 AM Chi Liu...

Hi, I am encountering similar problem, empty overlap set. I am very new to the metagenomics as well as racon. My commands are following, minimap2 -x map-ont 98ZLc_assembly_NP.fasta 98ZLc_nanopore_filt.fastq >...

Thanks for your kind reply. It has worked. Now i am getting slightly different following error, minimap2 -x sr 98ZLc_nanopore_assembly_racon_metka2.fasta 98ZLc_combineraw_IL.fastq > 98ZLc_mini_IL.paf racon 98ZLc_nanopore_raw.fastq 98ZLc_mini_IL.paf 98ZLc_nanopore_assembly_racon_metka2.fasta > 98ZLc_nanopore_assembly_racon_metka2_raconIL.fa [racon::Polisher::initialize]...

my bad. Thanks. But again some error so, anbu@bubulin:~/polyplax/downstreamanalysiswithvaclavassembly$ racon -t 19 98ZLc_combineraw_IL.fastq 98ZLc_mini_IL.paf 98ZLc_nanopore_assembly_racon_metka2.fasta > 98ZLc_nanopore_assembly_racon_metka2_raconIL.fa [racon::Polisher::initialize] loaded target sequences 1.175 s [racon::Polisher::initialize] loaded sequences 200.535 s [racon::Overlap::transmute] error:...

yes, it is !

thanks. so i made script.py file and saved it my working directory and ran the script. got the following error, anbu@bubulin:~/polyplax/downstreamanalysiswithvaclavassembly$ python script.py 98ZLc_combineraw_IL.fastq Traceback (most recent call last): File...

i have fastq file, is it ok