alienzj

Report here: https://github.com/bioconda/bioconda-recipes/issues/38110

Just uninstall kraken and/or krakenuniq using conda/mamba, then reinstall kraken2 + bracken using conda/mamba, **bracken-build** will work.

``` ─ Session info ────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────── setting value version R version 4.1.1 (2021-08-10) os Arch Linux system x86_64, linux-gnu ui RStudio language (EN) collate en_US.UTF-8 ctype en_US.UTF-8 tz Asia/Hong_Kong date 2021-10-12...

@xiangpin Thank you for your quick reply.  Nice! But I meet the second problem. ``` Error: Can't subset columns that don't exist. x Column `RareAbundanceBySample` doesn't exist. Run `rlang::last_error()`...

@xiangpin Many many thanks for your help. Now, I begin to enjoy the microbiome data exploration with MicrobiotaProcess. Thanks !

 @xiangpin Hi, I need your help~

The `mpse3_d0_bnt` is a MPSE object constructed with MetaPhlAn3 profile. I have no idea about `.abundance=?`. Could you please help me to solve this problem?

@xiangpin Hi, Shuangbin, thanks for your quick reply. After your meticulous and patient explanation, I began to gradually understand the processing logic of MPSE. I try to reproduce it ,...

I performed the LEfSe analysis using LEfSe python package, it can identified some biomarker. But when I use the `mp_diff_analysis`, cann't identify any biomarker, like above example. I did not...

@xiangpin Hi, Shuangbin, thanks for your reply. The above example can be reproduced.  Can I use `median` as `fc.method` ?