albustruong

ERROR: Offset around cut would be greater than reference sequence length.

2

**Describe the bug** CRISPResso version 2.2.14 [Command used]: /home/albustruong/miniconda3/envs/ngs/bin/CRISPResso -r1 2404-1-A4.R1.clean.fastq -r2 2404-1-A4.R2.clean.fastq --amplicon_seq ATTGCAGAGAGGCGTATCATTTCGCGGATGTTCCAATCAGTACGCAGAGAGTCGCCGTCTCCAAGGTGAAAGCGGAAGTAGGGCCTTCGCGCACCTCATGGAATCCCTTCTGCAGCACCTGGATCGCTTTTCCGAGCTTCTGGCGGTCTCAAGCACTACCTACGTCAGCACCTGGGACCCCGCCACCGTGCGCCGGGCCTTGCAGTGGGCGCGCTACCTGCGCCACATCCATCGGCGCTTTGGTCGGCATGGCCCCAT --prime_editing_pegRNA_spacer_seq GGAATCCCTTCTGCAGCACC --prime_editing_pegRNA_extension_seq CCTTTTCGCTAGGTCCTCGACGTCTTCCCTA --exclude_bp_from_right 0 --exclude_bp_from_left 0 -wc -3 --plot_window_size 10 -n Quinn -o...

Don't know how to export ggbio object to a figure

2

**Aim** To generate Manhattan plot with plotGrandLinear function **Script** library(GenomicRanges) library(ggbio) library(readr) library(tidyr) library(dplyr) input_dir = "/mnt/d/NGS/rna-seq/20240410/11_plot_prep" output_dir = "/mnt/d/NGS/rna-seq/20240410/13_manhattan_plot/ggbio" input_files

Hi Kishore, Thanks a lot for your effort building this tool. I've been trying to score a custom sequence using your provided script: *from keras.models import load_model from pkg_resources import...