Naturalist1986

fragment length parameter not working

1

Hi, I've run this command: iss generate --draft *.fna --abundance uniform -n 10000000 --output mix_bacteria_fungi_150 --cpus 128 --model MiSeq --fragment-length 150 --fragment-length-sd 20 But I'm still getting 300bp length reads:...

IndexError: sra-stat output parsing error!

3

Hi, I'm installed parallel-fastq-dump on a cluster using SLURM, but i get this error: (srr_download) moshea@moriah-gw-01:~$ parallel-fastq-dump --sra-id SRR2244401 --threads 4 --outdir . --split-files --gzip -T . 2024-01-07 12:31:15,300 -...

SAM file formatting

7

## Expected Behavior Hi, I'm trying to convert a SAM file created from a search result but I get this error: (mmseqs) moshea@shannon:/mnt/scratch$ samtools view -Sb SRR5234505.sam > SRR5234505.bam [E::sam_parse1]...

Abundance file shows more genomes than are used

2

Hi, I'm using this command to generate metagenomes from a set of genome fasta files: iss generate --draft (list of *.fna files) --output --model nextseq --coverage uniform -p 128 -n...