Yizhong Huang

As the genome of the pig data is 2.5G and the gtf file may be hundred M. So I should send it to you with an email-address.

> Human parameters are expected to work well for pig. I would not retrain for pig. > […](#) > On Fri, May 21, 2021 at 4:44 PM Yizhong Huang ***@***.***>...

Yes, I have done the BUSCO evaluation about my genome, rna-seq data (from the PASA) and homogoly protiens. The busco are 96%, 96%, 90%. But the final result which were...

Hi, thanks for your advices and I have runned the BUSCO about the Susscrofa reference assembly with the AUGUSTUS codes showed as follows (by chromosomes): ${Augustus}/bin/augustus --AUGUSTUS_CONFIG_PATH=${Augustus}/config --gff3=on --outfile=${outputfile}_augustus.gff --stopCodonExcludedFromCDS=false...

Hi, there, Sorry to disturb you! I have conducted the BUSCO of the Susscrofa reference assembly with the AUGUSTUS codes showed as follows (by chromosomes): ${Augustus}/bin/augustus --AUGUSTUS_CONFIG_PATH=${Augustus}/config --gff3=on --outfile=${outputfile}_augustus.gff --stopCodonExcludedFromCDS=false...

Ok, thanks! Yes, I have run the BRAKER for 2 days by using 8 cpus, the time for process of the augustus was long. Whether it is BRAKER1 or BRAKER2,...

Hi, Meiyuan I also used the BRAKER2 to do the gene prediction these days. When I run the gmes_petap.pl script, there have a problem, showing as follw: cannot find an...

> Hi, > can you please let me know which version of liftoff you are using? > Thanks! Hi, the version of the liftoff is v.1.6.1.  I have used...

Hi, the -chroms and the -unplaced file are listed as follows:   The features files : 

> has a database of the gff been created in your working directory? it should be the same name as the annotation file with "_db" at the end. if so,...