Junjie Chen
Junjie Chen
Thank you for your reply. Yes, it's amplified library. However, i have got the annot.fa(full length result), the cell number in full length result is too high. So, can i...
I mean i have extracted the cell barcode list from full length result. and i also have extracted cell barcode from the filtered barcode report to match the cell barcode...
Yes, i used GetFullLengthAssembly.pl script to get full length result and i found trust4 full length result may not filter other cell types. Therefore, i kept the cell barcodes of...
Thank you for your explanation. Only "barcode, cell_type, chain1, chain2, secondary_chain1, secondry_chain2" information shown in my barcode report file, so i have to run GetFullLengthAssembly.pl script to get full length...
it's amplified data. Curiously, the data whose candidate results are smaller is slower when processing reads.
I didn't try '--repseq' option. But the speed is really slow. 
i will try, thanks
Thank you for your answer. Is the annotator command? ![Uploading D7198A2A-DE9C-4f59-BD9D-910EB4A981DC.png…]()
Ok, i will try. Another question is how to calculate the umi for each contig? I found the umi count is equal to read count after converting barcode report to...