Biofarmer

Hi, I noticed that the input -1 and -2 should be paired and in order. If they are not ordered, I find that the megahit can still run without any...

Hi, may I ask a basic question about the header of contigs: For example: k119_20 flag=0 multi=10.0000 len=1000: k119_20 means that this contig is the 20th contig that were generated...

viral contigs abundance

1

Hi, thanks for your very useful tool, and the one I am using it version 1.9.0. I have extracted viral contigs from metagenomic sequencing data of stool, and now want...

using orf as input fasta for mmseqs easy-taxonomy

2

Hi, just a direct question that when the ORF fasta files (predicted by prodigal) is the input file with easy-taxonomy (MMseqs Version: 14.7e284), whether the fasta sequence will be again...

fastANI is not working

95

Hi Dr. Olm, I am using version 2.6.2, when running dRep compare with --S_algorithm fastANI, there is an error: Clustering Step 1. Parse Arguments Clustering Step 2. Perform MASH (primary)...

FPKM

2

Hi, thanks for your very useful tool, and the version I am working with is 0.6.1. One question about the coverm contig -m rpkm. As mentioned in the the link...

instrain profile step3

5

Hi Dr. Olm, I am using the inStrain v1.6.3 with code as below: inStrain profile file.sam reference.fna -o file.IS -p 24 -g reference.genes.fna -s reference.stb --database_mode --skip_plot_generation The output files...

FASTQ reads headers

2

Hi, I am using the version 3.2.2 of args-oap on paired-end shotgun sequencing files. As mentioned "If you use paired-end files, please make sure the forward/reverse reads end with _1...