Alexandre Thibodeau

Here is an uptade: Used mothur up to classification step. Deunique the sequences using Mothur Split by group using Mothur Removed "-" in the Mothur sequences using sed command on...

Silva 16S is unusable to classify my positive control 16S 2x 250 bp Illumina sequenced. Used Mothur up to OTU making Deunique sequenced split groups in the fastas, removed "-"...

Hi! I am just starting to explore 16s classification using Kraken2, but my feeling is that this is a classification tool, so you need to run the normal 16S pipeline...

I think Greengene2 wil be usefull only for specific tools as it is now in a tree form from what I can understand from it. Do not think it will...

RDP web do not exist anymore, therefore it is impossible to use it to fetch special database RDP for classifying 16S sequences.

Downloaded archea using and added Refseq bacteria (17 000 genomes, 21 Go compressed file)), manually, from NCBI new tool: NCBI Datasets (https://www.ncbi.nlm.nih.gov/datasets/). Needs 121 Go of free ram to build...

Database did build itself, took 1 hour but apparently did not use my bacteria genomes, only the archea. So I beleive it did not fin the fna. Maybe because the...