Ali Asgher Ali

Results 12 comments of Ali Asgher Ali

> Hi @skim245 , as far as I know, you have to build the raw loom file from your bam file first, in order to be able to retrieve the...

I have a simillar issue - I ran clustering, find markers etc. on a merged Seurat Object but do not know how to add spliced/unspliced information on the merged file....

Did anyone mange to resolve this? I am having the same issue

Just an FYI for others: I resolved this issue by copy pating my code directly into the terminal, using full addresses and not going through a script. Velocyto is now...

Here are the instructions :) https://mojaveazure.github.io/seurat-disk/articles/convert-anndata.html But there are people who have has errors with this (including myself)

Same here. My Seurata Object was an older version which I updated. ``` Creating h5Seurat file for version 3.1.5.9900 Adding counts for RNA Adding data for RNA Adding scale.data for...

Hello! I tried attempting this solution (see #13 ) but it did not work for me and produced a long traceback error. @cfayx1996 did yoz have any luck?

> Hi @AAA-3, > > I presume you are trying to filter your data for RNA Velocity? > > I tried to use this tutorial in for sorting in python,...

Hello, Yes, they are SCTransformed. `...` is just used to repeat the same steps eg: ``` Idents(h6_RQC)

I am also having this puzzle - did you solve it?