pod5-file-format
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nanoporetech
Pod5: a high performance file format for nanopore reads.
As discussed in: https://community.nanoporetech.com/posts/long-term-pod5-storage, is it possible to further compress POD5 losslessly for long term storage? Any thoughts on this would be much appreciated. Thanks!
It would be immensely helpful if this utility could be used to get the POD5 kit name from POD5 files via the CLI. This would enable a workflow for dorado...
## Multiple issues for pod5 conversion Hi, I have a dataset of fast5 reads that I am trying to convert to pod5 with the following command: ``` # path to...
## Issue Description I run the following command which finished successfully but the log says it merged 53 out of 73 files (73%). `pod5 merge -t 32 pod5/*.pod5 -o pod5merged/PAQ83015_bd9104da_d74a1b29.pod5...
Dear ONT, For POD5 files, I want to plot the raw signals with basecalled results in a region, e.g., 12-20 base-pairs. Is there a package or functions to extract the...
**Description:** I am using the following command to filter data from .pod5 files based on read IDs in a text file: **pod5 filter folder1 folder2 --output filtered.pod5 --ids read_ids.txt** When...
I am running pod5 convert fast5 on a sample with about 5000 fast5 files (from the sample samples, 4000 reads each), writing to a single pod5 per sample. - When...
## Description I am trying to convert an existing `.pod5` file that uses VBZ compression to a `.pod5` file using ZSTD compression using the `pod5 convert` CLI. For example, I...
Dear Nanopore-Team, Thank you for providing your work with us. My problem concerns a pipeline that includes multiple commands, as recommended in the Duplex documentation. I am working on long-read...
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Pod5: a high performance file format for nanopore reads.