kaizen89

I also have this error with 10X data: File "/usr/local/bin/velocyto", line 11, in sys.exit(cli()) File "/usr/local/lib/python3.6/site-packages/click/core.py", line 722, in __call__ return self.main(*args, **kwargs) File "/usr/local/lib/python3.6/site-packages/click/core.py", line 697, in main rv...

@RBBurl1227 yes, and for me the run command solved the issue. thanks

@SiyiWanggou As I understand, CB is a tag in the bam file which contains the barcodes. If you want to sort by cell barcodes then you should set -t CB....

How did you manage @RBBurl1227 ?

The run command as suggested by @gioelelm worked for me

@qiao0226 @yanwengong did you manage to find a solution?

I am really questioning the use of batch correction on multiple samples to run velocyto on the corrected matrices. Does anyone have any insight on this?

Same question here

Unfortunately the issue still persists. I tried to input the absolute path name and escaping the space and ( ) but I still get an error ``` salmon@salmon5820:/mnt/sda3/Dropbox (U932)/myDropbox/Lab/programs/Github_tools/ecotyper$ Rscript...

same here