Tony Zhou

I have the same problem and the folloing steps works with me(using R 3.4.1): `conda install bioconductor-clusterprofile` `conda install bioconductor-chipseeker` start R and enter: `remove.packages("GenomicFeatures")` quit R `conda install bioconductor-genomicfeatures`...

Hi Wendell, Sorry for the inconvenience, celescope's documentation really needs to be improved. Assume that you need to use the `rna ` pipeline. In celescope V1 version we need to...

Hi Wendell, I just created a repo for analyzing singleron RNA data based on nf-core scrnaseq best practice, hope you find this useful. https://github.com/zhouyiqi91/scrnaseq

https://discourse.scverse.org/t/metaclass-conflict-from-trying-to-import-scanpy/1133 You can try to downgrade matplotlib

> > https://discourse.scverse.org/t/metaclass-conflict-from-trying-to-import-scanpy/1133 You can try to downgrade matplotlib > Yeah, that's helps. However, I got an another error: ImportError: cannot import name 'is_categorical' from 'pandas.api.types'. I then downgraded pandas...

You forgot to add backslash at end of each line. https://unix.stackexchange.com/questions/368753/what-does-this-command-with-a-backslash-at-the-end-do#:~:text=A%20backslash%20escapes%20the%20next,command%20to%20span%20multiple%20lines.

It seems that the backslash was not properly added. You can try move all the arguments in one line to avoid this issue. For example ``` multi_rna --mapfile /hdd_a/andrea/Celescope/my.mapfile --genomeDir...

https://github.com/singleron-RD/CeleScope/blob/master/doc/chemistry.md singleron kit v1 corresponds to chemistry scopeV2.2.1, which requires at least 69bp R1. If you have 68bp R1, the last 1bp UMI will be thrown away. change `-soloUMIposition 0_57_0_68...

> https://github.com/singleron-RD/CeleScope/blob/master/doc/chemistry.md singleron kit v1 corresponds to chemistry scopeV2.2.1, which requires at least 69bp R1. If you have 68bp R1, the last 1bp UMI will be thrown away. change `-soloUMIposition...

if so，just remove the L1 and use U12 ---------- 该邮件从移动设备发送 --------------原始邮件-------------- 发件人："biozhao77 ***@***.***>; 发送时间：2024年3月5日(星期二) 晚上9:19 收件人："singleron-RD/CeleScope" ***@***.***>; 抄送："周义其 ***@***.***>;"Comment ***@***.***>; 主题：Re: [singleron-RD/CeleScope] BGI data was not suitable for version 2.0.7...