yuchen345 comments

Results 5 comments of


yuchen345

run match_cell_barcode, no error, no result, match_cell_barcode /data_RAGE_seq/data1 cell_barcode_stat.txt split_barcode.fastq flame_3M-february-2018.txt 2; split_barcode.fastq is zero,no other file generation。

Hi, Luyi, Would you please make an example of the usage of match_cell_barcode and explain the input files in more details? Is the fastq folder consisting of illumina sequencing data...

run match_cell_barcode, no error, no result, match_cell_barcode /data_RAGE_seq/data1 cell_barcode_stat.txt split_barcode.fastq flame_3M-february-2018.txt 2; split_barcode.fastq is zero,no other file generation。

Thanks for your reply! @LuyiTian Here is another error using sc_long_pipeline.py : > \### read gene annotation 2022-04-20 20:57:58 > > remove similar transcripts in gene annotation: Counter({'duplicated_transcripts': 370}) >...

run match_cell_barcode, no error, no result, match_cell_barcode /data_RAGE_seq/data1 cell_barcode_stat.txt split_barcode.fastq flame_3M-february-2018.txt 2; split_barcode.fastq is zero,no other file generation。

Thank you very much ! @LuyiTian More questions i am wondering: 1. As you said, the FLAMES searches for both directions and trims adapter sequence + cellbarcode/UMI at both directions,...

trimming only first adapter if multiple found, but not necessarily best alignment

@marcelm Hi, here is a similar question. "If a single read contains multiple copies of the same adapter, the basic rule is that the leftmost match is used for both...

trimming only first adapter if multiple found, but not necessarily best alignment

YES，they are. @marcelm I'd like to tirm off the `rightmost` ADAPTER and the following part. How to make it work? A completely novice user of Cutadapt and need your help.......