Shaojun Xie
Shaojun Xie
Hi there, Fastp is great! One thing I encounter is that some of the files doesn't have any reads if I use `--split`. Here is the command I used. ```...
Hi @lh3, I'm wondering whether there is a maximum number of bases for a reference genome. I'd like to know whether it's practical to build index on NCBI nt database....
Hi Manu, Thanks for sharing this data. I'm wondering whether you can also share the report. Then if I do some test I'll know whether my test is consistent with...
Hi, it seems that the `main` parameter is not working. Could you please fix it when you get a chance?
Hi, I'm wondering how high quality pairs are defined inside DRAGMAP? I got the info below? It seems that NO high quality pairs are identified for my read. The reads...
Hi , It seems that the latest version of `haslr` allows the user to provide multiple fastq files as input based on the code here: https://github.com/vpc-ccg/haslr/blob/0123746af93a9b0d79eee969510d12a88a9d6af3/bin/haslr.py#L300. This is not allowed...
Hi, thank you for developing the tool. Could you please let me know whether you have tried this tool on mouse data? Which vcf file do you recommend to build...
Hi @Xiaojieqiu, Because of the limitation of memory, Rstudio always crashes at the estimateDispersion step. In the cluster I got from Seurat, the number of cells varies a lot among...
In the link here: https://cole-trapnell-lab.github.io/monocle-release/tutorial_data/Olsson_dataset_analysis_final.html `plot_multiple_branches_heatmap` was used. I'm wondering how the `branches` was obtained from the results. Could you please give some explanation of how `c(3,4,5)` was defined? Which...
Thank you for the nice work flow published on F1000: https://f1000research.com/articles/9-1263/v1 Question 1: I am able to successfully run the R code upto the code below: ``` # split SCE...